Plasmid_Backbone

Part:BBa_K1321308:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)


pBla-Vhb-122-Bb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4667
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4667
    Illegal XhoI site found at 1709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4667
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4667
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4682
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 338
    Illegal AgeI site found at 178
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2798
    Illegal BsaI.rc site found at 4193


Design Notes

Z


Source

Y

References